Homogeneous mixtures of phenol and water at phenol to water mass ratios of 2. Phenol biodegradation by microorganisms is also influenced by temperature and microbe counts. Store the unused portion at 2 to 8c under nitrogen gas. All information recommendations and suggestions appearing herein concerning this product are based upon data obtained from the manufacturer andor recognized technical sources. Dec 07, 2006 this is a pretty silly question but how do i make water saturated phenol from phenol crystals. The top layer is a solution of phenol in water, and the bottom one a solution of water in phenol. Immediately flush eyes with excess water for 15 minutes, lifting lower and upper eyelids occasionally. Phenol bioultra, for molecular biology, tesaturated, 73% t synonym. Material safety data sheet west liberty university. A bottle of buffer is included for most dna applications, that.
Special exposure closed containers exposed to heat may explode. Picric acid, wetted 88 89 1 persistence and degradability not readily biodegradable in water. Toxic and hazardous industrial chemicals safety manual. We do use ph paper for quick spot checks on small volumes outlined below are two alternative methods for measuring ph of phenol.
To the best of our knowledge, the information contained herein is accurate. Material safety data sheet bromine water, saturated. Phenol saturated for molecular biology company name. Peg300 or peg400, rather than water, for immediate first aid treatment of dermal. The addition of 8hydroxyquinoline increases stability and colors the phenol yellow, so that the separation of the aqueous and organic phases is facilitated. Preparation of phenol for use in molecular biology applications is a timeconsuming and o.
Phenol is a colorless or white solid when it is pure. Ambion water saturated phenol is supplied premixed and at ph 6. Sigmaaldrich offers a number of phenol solution products. General description this phenol solution is a clear liquid saturated with citrate buffer. Determining the ph of water saturatedphenol solutions. To prepare activated charcoal slurry, mix thoroughly 50 g of activated charcoal in 400 ml about 2 cups water. Product and company identification product name phenol, saturated ph 4. Jul 01, 2014 information on phenol also known as carbolic acid and hydroxybenzene, for responding to chemical incidents. Use water spray, alcoholresistant foam, dry chemical or carbon dioxide. Acid phenol to solid phenol add rnasefree water until there is a layer of water on top of the phenol. Is there any method to prepare water saturated acidic phenol for rna extraction. Firstaid measures general advice show this safety data sheet to the doctor in attendance. Product and company identification phenol synonymsgeneral names.
Causes severe skin burns and eye damage toxic if inhaled suspected of causing genetic defects may cause damage to organs through prolonged or. Material safety data sheet phenol section 1 chemical product and company identification msds name. Please check the following link and pdf attachment. The halflives for phenol biodegradation in 15 river water samples averaged 4 and 7 days at 30 and 20c, respectively, but only about 20% 0. It is usually prepared by the hospital pharmacy because it is not commercially available in premixed liquid form. Test the ph periodically and do not use if the ph is water saturated phenol for rna extraction. Picric acid, wetted 88 89 1 ec50 other aquatic organisms 1 240 mgl algae. Phenol 108952 persistence and degradability readily biodegradable in water. Photocatalytic oxidation of benzene to phenol using. Phenol has a distinct odor that is sickeningly sweet and tarry.
Cool tanks drums with water spray and remove them to safety. Product and company identification phenol solution, 5% synonymsgeneral names. Phenol liquid and vapor pose a severe health hazard when inhaled or exposed to bare skin. Extinguishing use water spray, fog, foam, dry chemical, co or other agents as. Is there any method to prepare water saturated acidic. Accidental release measures personal precautions use personal protective equipment as required. Phenol is a benzene ring with one hydroxyl group substituted for a hydrogen atom. Molecular biology grade for nucleic acid purifications product description. The information provided in this material safety data sheet represents a compilation of data drawn directly from various sources available to us. State regulations california proposition 65 this product does not contain any proposition 65 chemicals. Water saturated phenol is used in rna and dna extraction procedures and is provided in one bottle of 400 ml.
If you try to dissolve more than this, you get two layers of liquid. Vapors can travel to a source of ignition and flash back. Fire fighting water should be contained if possible, as it is toxic and can cause environmental damage. The fume hood in our lab is filled with equipment so i cant. In case of skin contact wash off with soap and plenty of water. Making watersaturated phenol dec072006 hello all, this is a pretty silly question but how do i make watersaturated phenol from phenol crystals. Material safety data sheet phenol tris saturated sol. Phenol solutions can be used for a wide variety of molecular biology applications, including nucleic acid extractions using standard phenol chloroform protocols. Physical properties of phenol chemistry libretexts. Phenol united states environmental protection agency. Phenol saturated for molecular biology section 1 chemical product and company identification msds name. Phenol bioultra, for molecular biology, tesaturated, 73%. It is the users responsibility to determine the safety, toxicity and suitability. The buffer saturated phenol may be stored at 4c for 1 month for dna extraction.
Preparation of buffer saturated phenol for dna extraction. During fire, irritating and toxic gases of hydrogen chloride may be. Rinse thoroughly with plenty of water for at least 15 minutes and. First aid measures always seek professional medical attention after first aid measures are provided. Phenol standard, ppm persistence and degradability not established. How to make water saturated phenol for rna extraction. This safety data sheet has been prepared in accordance with the globally harmonized system for the classification and labeling of chemicals ghs. Test the ph periodically and do not use if the ph is phenol will be lost during the preparation of the buffer saturated phenol. Water cas no 7732185 material safety data sheet sds msds. Material is lighter than water, and so a fire can be spread by the use of water. Bp1751i400 synonyms buffered phenol solution recommended use laboratory chemicals 2. Call a physician or poison control center immediately. Identification of the substancemixture and of the companyundertaking.
None of the chemicals in this product are considered highly hazardous by osha. Phenol material safety data sheet cas no 108952 sdsmsds. Determining the ph of water saturated phenol solutions at severn biotech we use a ph probe to check phenol, which needs to be carefully rinsed after each measurement. In case of fire, use water, dry chemical, chemical foam, or alcoholresistant foam. Persistence and degradability picric acid, saturated persistence and degradability not established. Use water spray to keep fireexposed containers cool. Phenol is an organic compound appreciably soluble in water, with about 84. No component of this product present at levels greater than or equal to 0. Never give anything by mouth to an unconscious person. Phenol is used primarily in the production of phenolic resins and in the manufacture of nylon and other synthetic fibers.
Phenol is poorly soluble in water and, at room temperature, forms only a 6. Sigmaaldrich material safety data sheet date printed. Use water to cool nearby containers and structures. Thermo scientific pierce phenol, saturated is phenol supplied as a saturated solution ph 6. Lesser amounts of water will merely dilute the phenol and expand the area of exposure. Determining the ph of water saturatedphenol solutions at severn biotech we use a ph probe to check phenol, which needs to be carefully rinsed after each measurement. Phenol is both a manufactured chemical and a natural substance. The ph of phenol solutions is difficult to measure directly, although the ph of the buffer solution above the phenol is a good indication. A bottle of buffer is included for most dna applications, that require a higher ph of 7. I know i should just add phenol to the water until it no longer goes into solution but i want a ballpark ratio of phenol to water. Phenol water saturated solution,dnase,rnase,protease free,stab.
Wear full protective equipment, including suitable respiratory protection. You can taste and smell phenol at levels lower than those that are associated with harmful effects. Molecular biology grade for nucleic acid purifications. The fume hood in our lab is filled with equipment so i cant move in both the stirplate and weigh machine at the same time.
A high density shower is preferable to reduce phenol uptake. Read label before use obtain special instructions before use do not handle until all safety precautions have been read and understood. Next the polysomes are dissociated with sds and proteinase k and finally the protein is removed by several ph. I needed to prepare water saturated phenol for rna extraction. Preparation of buffer saturated phenol for rna extractions. Safety data sheet according to 29cfr19101200 and ghs rev. Making watersaturated phenol general lab techniques. Cardinal gracious road, chakala, andheri east, mumbai 400 099. The sodium salt of phenol, sodium phenoxide, is far more water soluble. Material safety data sheet phenol saturated for molecular biology section 1 chemical product and company identification msds name. Add enough deionized water to fill 100 g bottle to the base of the neck. To measure the ph of the phenol directly, ambion recommends mixing 2 ml of water saturated phenol with 5 ml of methanol and ml water to form a uniform solution, which can be measured with a ph meter. One more, the water saturated phenol will used for rna extraction. Do not use a straight stream of water, which will scatter and spread the fire.
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